Evaluation of CD80 and CD86 expression on apoptotic and nonapoptotic DCs following ATP treatment.

DCs were exposed to ATP for 30 minutes, washed, and incubated for an additional 6 hours. They were then labeled with the PE-conjugated anti-CD80 (B7.1) or anti-CD86 (B7.2) mAb before the detection of apoptosis by TUNEL (FITC). The dot plots show the CD80 (A) or CD86 (B) and TUNEL double labeling of control cells (Ct) (left panel) and those treated with 5 mmol/L ATP (ATP) (right panel).