Super-shift assay with HAF antibody and Hep3B nuclear extract.

(A) Western blot showing the specificity of HAF antibody utilized in the supershift assay. Arrows indicate the two proteins discussed in the text. (B) Increasing amounts (1, 5, and 10 μL) of anti-HAF serum were incubated with Hep3B nuclear proteins (5 μg) in the presence of the EP17 probe (lanes 2-4). Lane 1 is a control reaction with nuclear proteins alone. Control reactions with preimmune serum in the presence (lane 5) or absence (lane 6) of nuclear extract. Imm. Serum and P.I. Serum refers to immune and preimmune serum respectively, N.E. refers to nuclear extract. S, NS, and SS represent specific, nonspecific, and super-shifted complexes, respectively.