Northern analysis of HAF expression in different tissues and at different development stages in liver and kidney.

The probe used was 700 bp (nt 1-700) of HAF cDNA. Total RNA from NN10 cells was used as a positive control. (A) A 2.6-kb single transcript was identified in all the tissues indicated above each lane with the exception of the liver. The bottom panel shows ethidium bromide stained 28 S rRNA. (B) Expression of HAF in liver at indicated development ages. The bottom panel shows ethidium bromide stained 28 S RNA. (C) Quantitative analysis of panel B using densitometer scanning. Scanner units were normalized for 28 S RNA. (D) Expression of HAF in kidneys at different development stages. The arrow indicates 2.6-kb HAF mRNA in upper panel and 28 S rRNA in bottom panel.