Fig. 4.
Cell cycle entry of UC T cells is not affected by the purification process.
Nonpurified or purified UC T cells (Dynal) were stimulated with immobilized anti-CD3 and anti-CD28 antibodies. Cell cycle entry was monitored at 0, 2, and 3 days by assessing DNA content of PI-stained cells on a FACScan cytometer. The percentage of cells in the S and G2/M phases of the cell cycle are indicated and results are representative of data obtained in 1 of 5 representative experiments.
