Fig. 5.
Induction of an active β2 integrin conformation by VCAM-1–Fc binding to VLA-4.
Myelomonocytic HL60 cells were pretreated with medium (thin line) or soluble VCAM-1–Fc (solid line) (40 μg/mL) for 30 minutes at 37°C. After washing, fluorescence-activated cell sorter analysis was performed as described in “Materials and methods” using mAbs anti-αL (L15) and anti-αM (Bear-1), which both detect the respective integrin independent of its activation state, as well as mAb CBRM1/5 (anti-αM), which only binds to the activated αM chain of Mac-1.28 The broken line represents the nonspecific control mAb.
