American Society of Hematology

Fig. 2.

$Fig. 2. Analysis of blood leukocytes from lys-EGFP-kimice. / (A-D) FACS analysis of blood samples collected from the tail veins. Red cells were lysed to obtain the leukocyte fraction. After suspension in PBS, the samples were analyzed for GFP fluorescence, and the data were plotted as relative fluorescence intensity (Y axis) versus the relative cell number (X axis). The numbers in each panel represent the percentage of fluorescent-positive cells in the respective sample. The following animals were used: (A) control mice; (B) heterozygous lys-EGFP mice, not neo-deleted; (C) heterozygous lys-EGFP mice, neo-deleted; (D) homozygous lys-EGFP mice, neo-deleted. The following average fluorescence intensities plus or minus SD were calculated from 4-5 animals in each group: (A) less than 2, (B) 92.6 ± 51.5, (C) 194.6 ± 50.1, and (D) 625 ± 396.3. (E) Genotypic analysis. Genomic DNA was prepared from mouse tails and analyzed by PCR using primers that amplify a 220-bp fragment from the wild type allele and a 680-bp fragment from the targeted allele. (F) Micrographs of a fresh blood sample from a lys-EGFP+/kimouse were made using phase contrast (left) and fluorescence microscopy (middle). The picture shown on the right was obtained by overlaying the 2 images. (Scale bar, 10 μm.)$

Analysis of blood leukocytes from lys-EGFP-kimice.

(A-D) FACS analysis of blood samples collected from the tail veins. Red cells were lysed to obtain the leukocyte fraction. After suspension in PBS, the samples were analyzed for GFP fluorescence, and the data were plotted as relative fluorescence intensity (Y axis) versus the relative cell number (X axis). The numbers in each panel represent the percentage of fluorescent-positive cells in the respective sample. The following animals were used: (A) control mice; (B) heterozygous lys-EGFP mice, not neo-deleted; (C) heterozygous lys-EGFP mice, neo-deleted; (D) homozygous lys-EGFP mice, neo-deleted. The following average fluorescence intensities plus or minus SD were calculated from 4-5 animals in each group: (A) less than 2, (B) 92.6 ± 51.5, (C) 194.6 ± 50.1, and (D) 625 ± 396.3. (E) Genotypic analysis. Genomic DNA was prepared from mouse tails and analyzed by PCR using primers that amplify a 220-bp fragment from the wild type allele and a 680-bp fragment from the targeted allele. (F) Micrographs of a fresh blood sample from a lys-EGFP⁺/kimouse were made using phase contrast (left) and fluorescence microscopy (middle). The picture shown on the right was obtained by overlaying the 2 images. (Scale bar, 10 μm.)

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