American Society of Hematology

Fig. 5.

$Fig. 5. Morphology of EGFP+ cells sorted from the bone marrow of lys-EGFP+/ki mice. / Bone marrow cells were prepared and sorted by FACS. (A) FACS profile showing relative fluorescence intensity of GFP+ cells versus forward scatter, a parameter that is proportional to the cell's volume. The profile was subdivided by gating into a highly positive fraction (2 × 103 to 1 × 104, top quadrant), a moderately positive fraction (4.5 × 101 to 2.3 × 102, middle quadrant), and a negative fraction (100 to 101, bottom quadrant). (B) Cell profiles are shown from these 3 gated areas after sorting. (C) Micrographs of the sorted cells stained with Diff-Quik (a May-Grünwald Giemsa–like stain).$

Morphology of EGFP⁺ cells sorted from the bone marrow of lys-EGFP⁺/ki mice.

Bone marrow cells were prepared and sorted by FACS. (A) FACS profile showing relative fluorescence intensity of GFP⁺ cells versus forward scatter, a parameter that is proportional to the cell's volume. The profile was subdivided by gating into a highly positive fraction (2 × 10³ to 1 × 10⁴, top quadrant), a moderately positive fraction (4.5 × 10¹ to 2.3 × 10², middle quadrant), and a negative fraction (10⁰ to 10¹, bottom quadrant). (B) Cell profiles are shown from these 3 gated areas after sorting. (C) Micrographs of the sorted cells stained with Diff-Quik (a May-Grünwald Giemsa–like stain).

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