Cotransfection of a c-myc antisense expression vector ablates rescue of WEHI 231 cells from anti-IgM–mediated apoptosis by A-Myb.

WEHI 231 cells were transiently transfected by electroporation with 10 μg pON407 β-Gal expression vector in the presence of 10 μg either pLL1 or pECE, empty vector, in combination with 10 μg of either pOPRSV-as-c-myc or pOPRSV parental vector. Cultures were either left untreated (white bars) or treated 6 hours after transfection with 2 μg/mL anti-IgM antibody for an additional 24 hours (black bars). Cellular lysates were subjected to β-Gal assay and absorbance expressed as percentage of untreated cells transfected with control vectors alone (lane 1). The mean and SD are representative of 2 separate experiments, each carried out in duplicate.