In situ zymography and immunohistochemical staining of brain sections

. In situ zymography in panels A-F; panels A and D are developed without plasminogen, and all other panels are developed with plasminogen. Panels C and F also contain anti-tPA antibodies. The white arrows indicate the area of the infarct (magnification × 3). (G) Immunohistochemical staining of tPA 6 hours after reperfusion. The black arrows indicate tPA-positive blood vessels. (H) Immunohistochemical staining of uPA in the area of penumbra 72 hours after reperfusion (magnification in G and H × 400).