Immunohistochemical staining of laminin

. Panels A and B show normal cortex in an animal without stroke. Panel A was developed with anti-laminin but without pretreatment in vitro of the section with proteinase. Panel B is an adjacent section with in vitro proteinase treatment. Panels C to F are from stroked animals and developed with antilaminin and no proteinase treatment. Panels C and D are 10 minutes after reperfusion and panels E and F are 6 hours after reperfusion. Panels C and E represent PBS-treated and panels D and F neuroserpin-treated animals (magnification × 100).