Fig. 3.
Sustained expression of the GFP reporter gene in long-term reconstituted mice.
Peripheral blood of 4 long-term reconstituted animals from 2 independent experiments was assayed at the indicated time points for the persistence of GFP expression over 5 to 6 months. A total of 13 of 13 animals showed sustained long-term expression with little change in the percentage of GFP-expressing cells. The number of transplanted, GFP+ donor cells per animal are indicated. All donor cells were mixed with 2 × 105 recipient-type (Ly-5.1) marrow cells prior to transplantation.
