American Society of Hematology

Fig. 6.

$Fig. 6. LT-HSC remain GFP+ in significantly higher proportion than developing bone marrow cells. / Two mice reconstituted for 10 months were analyzed for GFP expression in developing bone marrow cells and within the hematopoietic stem cell population using 5-color FACS analysis. Cells were stained for the donor marker (Ly-5.2, allophycocyanin), lineage markers (Cy5-PE), c-kit (PE), Sca-1 (Texas Red), and GFP (fluorescence in the fluorescein isothiocyanate channel). The numbers in parentheses indicate gating of lineage-marker negative-to-low cells according to the numeric scale given on the top of the lineage-marker histogram. Donor-derived, LT-HSC will be present at high purity in the Ly-5.2+, Sca-1+, c-kit+, Lin (0-80) gated population. The entire hematopoietic stem cell pool, which includes short-term reconstituting stem cells, will be present in the Lin (0-100) fraction.$

LT-HSC remain GFP⁺ in significantly higher proportion than developing bone marrow cells.

Two mice reconstituted for 10 months were analyzed for GFP expression in developing bone marrow cells and within the hematopoietic stem cell population using 5-color FACS analysis. Cells were stained for the donor marker (Ly-5.2, allophycocyanin), lineage markers (Cy5-PE), c-kit (PE), Sca-1 (Texas Red), and GFP (fluorescence in the fluorescein isothiocyanate channel). The numbers in parentheses indicate gating of lineage-marker negative-to-low cells according to the numeric scale given on the top of the lineage-marker histogram. Donor-derived, LT-HSC will be present at high purity in the Ly-5.2⁺, Sca-1⁺, c-kit⁺, Lin (0-80) gated population. The entire hematopoietic stem cell pool, which includes short-term reconstituting stem cells, will be present in the Lin (0-100) fraction.

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