AF4 is required for normal thymocyte expansion.

Thymus from mAF4−/− glucocorticoid-treated mice show a decrease in the reconstitution of the number of DP cells. (A) Kinetic analysis of thymocyte reconstitution. T-cell counts after glucocorticoids in mutant (•) and control (□) mice over a 2-week period. Data are representative of 6 control mice and 6 mutant mice except when indicated. Two-tailed Student P value is shown, where significant. **P < .05; ***P < .001. (B) FACS analysis was performed on thymocytes of control and mutant mice at day 0 (d0), day 9, and day 12 after the glucocorticoid injections as indicated in “Materials and methods.” T-cell maturation was assayed using the CD4 and CD8 markers. Results shown are representative of 6 separate experiments.