Analysis of bone marrow hemopoiesis.

(A) Representative flow cytometry analysis of bone marrow B cell. Absolute number of B220⁺ cells in the bone marrow of mAF4 mutant mice and controls. Cell counts are given (×10⁶). (B) and (C) Representative flow cytometry analysis of bone marrow B-cell–stained populations from 3-week-old mAF4−/− and wild-type mice; cell counts (×10⁶) are indicated in the corresponding quadrant. Mature, immature, and transitional B cells are boxed. Staining was performed with B220, IgM, and CD43. (D) Total B cell numbers in the bone marrow of mAF4 mutant and wild-type mice. The data are the mean of 6 mAF4 null mice and of an equal number of wild-type littermates. The relative SD is shown. For statistical analysis, see Table 1.