Analysis of fusions containing a single predicted transmembrane domain.

Phosphorimage of the 7 constructs containing a single TMD analyzed by SDS-PAGE is shown. In vitro translations were done in the presence (+) or absence (-) of canine pancreatic rough microsomes (RMs). Endo H treatment was done in the presence (+) of 0.5% CHAPS, while proteinase K treatment was done in the presence (+) or absence (-) of 0.5% CHAPS. Glycosylated products are indicated by circles, while proteinase K–resistant fragments are indicated by asterisks.