Analysis of fusions containing adjacent pairs of predicted transmembrane domain.

Phosphorimage of the 3 constructs containing adjacent pairs of TMDs analyzed by SDS-PAGE is shown. In vitro translations were done in the presence (+) or absence (-) of canine pancreatic RMs. Endo H treatment was done in the presence (+) of 0.5% CHAPS, while proteinase K treatment was done in the presence (+) or absence (-) of 0.5% CHAPS. Glycosylated products are indicated by circles, while proteinase K resistant fragments are indicated by asterisks.