Fig. 3.
Evaluation of myeloid differentiation in mutant FdCP1 cells.
(A) Histologic analysis. FdCP1-derived cell lines expressing pSV2-neo alone (19-neo; A, B), FLAG-Stat5b WT (19-WT; C, D), FLAG-Stat5bm/m (19-M1 and 19-M2; E-H) were stained with Wright–Giemsa before or after differentiation in GM-CSF for 3 days. These photomicrographs are representative of the results obtained from 2 independent experiments. (B) Flow cytometric analysis. 19-neo, 19-WT, 19-M1, and 19-M2 cells were evaluated for expression of F4/80 (left column) or side scatter (SSC; right column) before (light tracing) or after differentiation (dark tracing) in GM-CSF for 3 days. These studies were carried out on the same set of cells evaluated by Wright–Giemsa staining. These histograms are representative of several independent experiments.