American Society of Hematology

Fig. 5.

$Fig. 5. Northern blot analysis demonstrating expression of Stat5 target genes. / RNA was prepared from FdCP1 (lanes 1, 2), Ba/F3 (lanes 3, 4), 19-M1 (lanes 5, 6), and 19-M2 (lanes 7, 8) cells before (−) or after (+) stimulation with IL-3 for 30 minutes. Thirty-five micrograms total RNA was fractionated on a formaldehyde gel and sequentially hybridized with a CIS-1 (top panel), OSM 1 (middle panel), and β-Actin (bottom panel) probe. Quantitation of signal intensity was measured on a Phosphor-imager.$

Northern blot analysis demonstrating expression of Stat5 target genes.

RNA was prepared from FdCP1 (lanes 1, 2), Ba/F3 (lanes 3, 4), 19-M1 (lanes 5, 6), and 19-M2 (lanes 7, 8) cells before (−) or after (+) stimulation with IL-3 for 30 minutes. Thirty-five micrograms total RNA was fractionated on a formaldehyde gel and sequentially hybridized with a CIS-1 (top panel), OSM 1 (middle panel), and β-Actin (bottom panel) probe. Quantitation of signal intensity was measured on a Phosphor-imager.

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