Expression of PN-I activity in

E coli and PN-I immunoblot analysis. (A) The specific activity of the expressed PN-I (●) is plotted as a function of growth of BL21 cells transformed with the recombinant plasmid ○. (B) Antiserum raised against peptide KSSVRIKNPTRVEEC is incubated at 1:1500 dilution with blotted pure wild-type PN-I (lane 1); antiserum against peptide DNSNIILLGDSQGDC is incubated at 1:1500 dilution with blotted pure wild-type PN-I (lane 2), and blotted extracts of BL21 cells transformed with nonrecombinant (lane 3) and recombinant plasmid (lane 4).