Activation of reporter gene expression in transiently transfected HepG2 hepatoma cells by CPX.

HepG2 cells were cotransfected with the indicated luciferase reporter gene constructs together with a β-galactosidase control expression vector. Following splitting and stimulation with hypoxia (1% oxygen) and/or CPX (8 μmol/L) for 43 hours, reporter gene activities were determined and expressed as a ratio between luciferase and β-galactosidase activities. The luciferase constructs contained 3 wild-type HBSs (pGLHIF1.3) or 3 mutant HBSs (pGLHIF1mt.3) as described previously.13 The empty parental vector pGL3 promoter was included as control. Means ± SD of 3 independent experiments.