Fig. 7.
Iron and aluminium block the DFX- and CPX-mediated reporter gene induction in HRCHO5 cells.
The cells were stimulated with the optimal DFX (100 μmol/L) and CPX (8 μmol/L) concentrations, as determined in Figures 1 and 2, respectively, for 18 hours under normoxic (20% oxygen) and hypoxic (1% oxygen) conditions. The indicated concentrations of ferrous ethylenediammonium sulfate (A) and AlCl3 (B) were added at the beginning of the experiment. After preparation of cell extracts and determination of the luciferase activities and protein contents, the results were expressed as luciferase activities in relative light units per microgram of cellular protein. Means ± SD of 3 independent experiments.
