Expression of hEBF is restricted to B-cell–derived cell lines.
(A) RT-PCR analysis to detect expression of hEBF in a panel of cell lines representing different lineages and B-cell developmental stages, as indicated. PBL indicates normal human peripheral blood cells, and − indicates a negative control with an RT reaction in which no RNA has been added. The upper part shows an ethidium bromide–stained agarose gel with the resulting GAPDH PCR products after 25 cycles of PCR with cDNA from the indicated cell lines. −RT indicates that RNA preparations have been included in the reaction without prior cDNA synthesis. The lower panel shows an autoradiogram of EBF RT-PCR products blotted to a nylon membrane after 30 cycles from the same cDNA preparations and hybridized with an internal oligonucleotide. (B) Autoradiograms of EMSA using approximately 5 μg of nuclear extracts from a panel of cell lines and either a32P-labeled consensus Oct protein-binding site or the EBF-binding site from the human mb-1 promoter as probes. F indicates free probe.
