hEBF is expressed during normal B-cell development.

RT-PCR analysis of sorted primary human cells from either bone marrow or peripheral blood, as indicated. GAPDH message was detected after 27 cycles of PCR, blotting of the resulting products to a nylon membrane, and hybridization to an internal oligonucleotide; whereas 32 cycles of PCR were performed before blotting and hybridization to detect EBF-encoding mRNA. −RT indicates that RNA preparations have been included in the reaction without prior cDNA synthesis.