hEBF and E47 collaborate to induce functional activity of the 14.1 promoter.

(A) The relative luciferase activity after transient transfection of 250 ng of a 14.1 promoter-controlled reporter gene into epitheloid HeLa cells together with increasing amounts (100 or 600 ng) of hEBF expression plasmid (black bars). The gray bars indicate the activity of the fos promoter-controlled reporter construct. (B) Induction of either a 14.1 black bars or λ5white bars promoter-controlled luciferase reporter gene after transfection with either empty, hEBF-, E47-, or the combination of EBF- and E47-encoding expression plasmids, as indicated. The data in both panels were collected from 4 transfections in which the amount of DNA was normalized by the addition of empty expression plasmid (cDNA3). Error bars indicate standard deviation.