PML and NPM immunofluorescence (IF) in t(5;17) cases and cell-line controls.

PML immunofluorescence using the PG-M3 antibody shows a wild-type pattern (discrete nuclear dots) in HL60 and in both patients, and a microparticulate diffuse nuclear pattern in the t(15;17) NB4 cell line. NPM immunofluorescence using the NA24 antibody shows a wild-type nucleolar pattern in both cell lines and in theNPM-RARα–negative patient (case 55), and a diffuse nuclear pattern in the NPM-RARα–positive patient (case 54). Images were captured with a Leica TCS NT confocal microscope.