Lysis of MMB3.19 target cells by in vitro restimulated CTLs.

(A) CD4-enriched lymph node cells from MMB3.19-primed wt,pfp^o, and gld mice were restimulated in vitro with irradiated MMB3.19 cells in the presence of T-STIM. They were then used as effectors in a JAM assay with³H-TdR–labeled MMB3.19 target cells. (B) CD8-enriched splenocytes from MMB3.19-challenged wt,pfp^o, and gld mice were restimulated in vitro with irradiated MMB3.19 cells in the presence of T-STIM. They were depleted of NK cells and CD4⁺ T cells prior to use as CTL effectors against ³H-TdR labeled MMB3.19 targets. The data are presented as the percentage of specific lysis plus or minus one standard deviation.