RD114-pseudotyped vector particles efficiently transduced cells capable of establishing multilineage hematopoiesis in immunodeficient (NOD/SCID) mice.
The 3 rows show the FACS profile of bone marrow cells obtained from NOD/SCID mice killed 8 weeks after receiving in vitro–expanded human CD34+ cells (1.0 × 105 input cells). The cells were stained with a human-specific CD45 antibody and analyzed for human engraftment. The gated human CD45+ cells from these animals were analyzed for human CD19 (B-lymphoid) or CD33 (myeloid) expression. Each row shows the FACS profile of bone marrow cells obtained from a NOD/SCID recipient of RD114-transduced cord blood CD34+ cells. The bone marrow from the animals shown was negative for RCR by PCR analysis (data not shown).
