Construct encoding the SS2 fusion toxin gene fragment used in these studies.

A Nco 1/HindIII gene fragment was cloned by PCR and splice overlap extension encoding DT₃₉₀, a 5 amino acid connector, the downstream anti-CD3 sFv derived from the 11452C11 hybridoma, and a cysteine residue on the c-terminus. The gene was cloned into the pET21d expression vector forming the plasmid pDTmCD3sFv.CYS.pET21d.