A representation of the detection of dCK mRNA expression by RT-PCR analysis in leukemic blasts from patients with resistant AML.

For each sample, at least 3 PCR amplifications were performed. T7B6 dCK-PCR constructs were separated on a 1.5% agarose gel and visualized by ethidium bromide staining. Indicated are dCK-PCR constructs amplified from purified leukemic blasts from patients with resistant AML and PHA-stimulated T cells from the same individuals.