Coupled in vitro transcription-translation test.

³H-leucine–labeled dCK proteins were synthesized and analyzed on a 12.5% SDS-PAGE gel. The dCK protein generated from a dCK-PCR T7B6 construct from healthy donor PHA T cells (wt), from a T7B6 construct with a deletion of exon 5 (del 5; rAML6), from a T7B6 construct with a deletion of exons 3 to 4 (del 3-4; rAML3), and from a T7B6 construct with a deletion of exons 3 to 6 (del 3-6; rAML4).