Fig. 4.
Resistance to AraC was introduced by in vitro exposure to increasing concentrations of AraC.
(A) Expression of dCK mRNA determined by RT-PCR analysis in rat leukemic cells during the course of induction of AraC resistance in vitro. Cell samples at different concentrations of AraC (in moles per liter) were obtained from exponentionally growing cells with more than 90% viability. The cells at 10−5-mol/L AraC were defined as the RO/1-A cell line. (B) Corresponding dCK activities in nmol/min × mg of protein during induction of AraC resistance in vitro as determined on in vitro–translated dCK proteins of the total PCR reaction.
