Fig. 3.
Fig. 3. Separation of the 34Leu and 34Val activation peptides on reverse-phase HPLC. / Purified FXIII 34Val (0.9 nmol/0.18 mL), FXIII from a mixed genotype (34Val/Leu; 0.5 nmol/0.18 mL), and FXIII 34Leu (0.06 nmol/0.02 mL) were analyzed by HPLC after activation by 10 U/mL human α-thrombin for 1 hour at 37°C. Peptides were detected by absorbance at 205 nm.

Separation of the 34Leu and 34Val activation peptides on reverse-phase HPLC.

Purified FXIII 34Val (0.9 nmol/0.18 mL), FXIII from a mixed genotype (34Val/Leu; 0.5 nmol/0.18 mL), and FXIII 34Leu (0.06 nmol/0.02 mL) were analyzed by HPLC after activation by 10 U/mL human α-thrombin for 1 hour at 37°C. Peptides were detected by absorbance at 205 nm.

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