Fig. 4.
![Fig. 4. Activation of FXIII 34Val and FXIII 34Leu by human -thrombin as analyzed by HPLC. / (A) FXIII (4.0 μmol/L) was incubated with increasing doses of thrombin for 1 hour at 37°C. (B) FXIII (4.0 μmol/L) was incubated with 0.5 U/mL thrombin at 37°C for increasing periods of time. Squares represent FXIII 34Val and circles represent FXIII 34Leu. Results shown for each experiment are mean values ± SD of 3 replicates. Release of the activation peptides was expressed as the ratio between the molar quantity of the peptides ([AP]) and molar quantity of the peptides at full activation ([AP]f).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/3/10.1182_blood.v96.3.988/5/bloo01557004x.jpeg?Expires=1735854168&Signature=RKEec7Z1CT7zgBWPET-hh6M2fwcbv~WV-evlQLdTIJZekfzUcUyBKApxEux6zhOQeDnfazDkw2UB5qlIQ~MnH1FkP9rTEbLWvUC76M9eKYSSzLjZcucI645JJnk88UCGhjEAfM97QRXmdmaRg7VXFsHr6W6CVB4ds9~SPp~cOgEc5bxRpetj2uu9DyCU4FkuYAnuI42flvJMOsYwok8KTHtm0S2UqESSBvjF~Qtu~ieptW4TduiQpguc1RwicaZU7~cmbdh0bNOeT6QDLSktZ0ceAN56yAA4s93eIkvmzO9OPJ8o0sCyHnHmhaG~ZLhvPZuPLlfLGHJRMYsFJASq8g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of FXIII 34Val and FXIII 34Leu by human -thrombin as analyzed by HPLC.
(A) FXIII (4.0 μmol/L) was incubated with increasing doses of thrombin for 1 hour at 37°C. (B) FXIII (4.0 μmol/L) was incubated with 0.5 U/mL thrombin at 37°C for increasing periods of time. Squares represent FXIII 34Val and circles represent FXIII 34Leu. Results shown for each experiment are mean values ± SD of 3 replicates. Release of the activation peptides was expressed as the ratio between the molar quantity of the peptides ([AP]) and molar quantity of the peptides at full activation ([AP]f).
