Fig. 5.
![Fig. 5. Time-course of activation of FXIII by thrombin in the presence of polymerizing fibrin. / The activation of 3.1 μmol/L purified FXIII 34Val (squares) and FXIII 34Leu (circles) by 0.2 U/mL human α-thrombin with the addition of 2.7 μmol/L fibrinogen was analyzed by HPLC. Diamonds represent fibrinopeptide A and triangles fibrinopeptide B. Release of the peptides was expressed as the ratio between the molar quantity of the peptides ([peptide]) and molar quantity of the peptides at full activation ([peptide]f). Results shown are mean values ± SD of 4 experiments. The inset shows a typical chromatogram with 4 defined peaks for fibrinopeptide A, fibrinopeptide B, FXIII 34Val activation peptide, and FXIII 34Leu activation peptide.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/3/10.1182_blood.v96.3.988/5/bloo01557005y.jpeg?Expires=1735853406&Signature=28kdcA~sj7yVI8eP~S3ZtlJzUU4~mYjY6peDDzCW8C0qarLmkr3olM0Kf8gGv2znq10u6MjTt6IlQb6~u41WQ2HW4Q4CfxtU-b3UFwisvOUUWsXEdiijJs5zN-aHlvpS0LHLu8weD~u~B0Hpc13-jWzhboyNs6fLIJsx~7jbzoXQlDBRJNeHUEFfCLzvkXLSEHMGirC~boosSwKiZwQ~46HWyoJueUQ8RoxQA1zWDuGqolyeuKCFCZc7UpG0RvzfC0vMXKR0ZEtjjEJ0UIV7T4mKhvip0z6XOlTAb59yfAlJ8b5NtlzKoDCFz8CXuF1leiI1naEH4A2WuRhImIQ2qw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Time-course of activation of FXIII by thrombin in the presence of polymerizing fibrin.
The activation of 3.1 μmol/L purified FXIII 34Val (squares) and FXIII 34Leu (circles) by 0.2 U/mL human α-thrombin with the addition of 2.7 μmol/L fibrinogen was analyzed by HPLC. Diamonds represent fibrinopeptide A and triangles fibrinopeptide B. Release of the peptides was expressed as the ratio between the molar quantity of the peptides ([peptide]) and molar quantity of the peptides at full activation ([peptide]f). Results shown are mean values ± SD of 4 experiments. The inset shows a typical chromatogram with 4 defined peaks for fibrinopeptide A, fibrinopeptide B, FXIII 34Val activation peptide, and FXIII 34Leu activation peptide.
