Fig. 3.
![Fig. 3. Expression of Jk proteins in transcription-translation coupled reticulocyte lysate system. / (A) Autoradiogram ofl-[35S]-methionine-labeled proteins wt Jk, Jk(S291P), and Jk(N211I) in vitro synthesized from pT7TS-wt Jk, pT7TS-Jk(S291P), and pT7TS-Jk(N211I) vector constructs, respectively, and analyzed by SDS-PAGE. Transcription-translation reactions were done with or without canine pancreatic microsomal membranes as indicated. The pT7TS vector alone was used as negative control. Arrows on the right refer to size of products (kd).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/4/10.1182_blood.v96.4.1566/4/h81600003003.jpeg?Expires=1735577031&Signature=W4chmRjBrD5Gvp3OXTCX~WJURuybaTSQxoPr6iTYLtApwTdSLdZegPuzAOZX8sZT0YgBNA5OkEKY60sIpzU~0seW7UR2AsjCXaLIgSFo0-kjDYHhN6hZZgb-y1sLTnrCHKf-i6T8NenHwxHAZt4VdhFU7SIrgPMoDj~TPgoW1RozdrdGmnPJ9n9oCsZ0S3b-25uZBTbfezbwECMRNQbfvDxSG88OiNKrrfSDIlVb9ZF9Tg4j~e1WaOxiZULTFpniU-cK~9hvKcvSOhPbfbPGvDYILaevo3s8Vj~0Q3MLD3qMcrMGNaPcDHHSBZUX0Ww0K0IPKv6aBSkA6ey88ylKmg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of Jk proteins in transcription-translation coupled reticulocyte lysate system.
(A) Autoradiogram ofl-[35S]-methionine-labeled proteins wt Jk, Jk(S291P), and Jk(N211I) in vitro synthesized from pT7TS-wt Jk, pT7TS-Jk(S291P), and pT7TS-Jk(N211I) vector constructs, respectively, and analyzed by SDS-PAGE. Transcription-translation reactions were done with or without canine pancreatic microsomal membranes as indicated. The pT7TS vector alone was used as negative control. Arrows on the right refer to size of products (kd).
