Expression of wt Jk and Jk(S291P) polypeptides in K562 transfectants.

K562 cells transfected with pCEP4-wt Jk or Jk(S291P) cDNA constructs were stained with the human monoclonal antibody (MoAb) anti-Jk^a (1:2 dilution) used as control (light curve) and the human MoAb anti-Jk^b (diluted 1:2 in phosphate-buffered saline) (heavy curve), followed by PE-goat antihuman IgG (Fab′2 fragments) and analyzed by flow cytometry. The log of the fluorescence intensity is shown on the abscissa and the cell number on the ordinate. The geometric means were reported for each population analyzed.