Effects of neutralizing antibody to VCAM-1 and α₄β₁-integrin on TRAP-positive multinucleated osteoclast formation in the co-cultures of 5TGM1 myeloma cells and primary mouse bone marrow cells.

A mixture of 5TGM1 cells (1 × 10³) and primary mouse marrow cells (1 × 10⁶) in suspension was inoculated in 48-well plates and cultured with or without 10 μg/mL of anti–VCAM-1 antibody (VCAM-1 Ab), anti–α₄β₁-integrin antibody (α₄β₁ Ab), anti–ICAM-1 antibody (ICAM-1 Ab), or control IgG. After 6 days of culture, cultures were fixed and the number of TRAP-positive multinucleated osteoclasts was determined. Data are expressed as mean ± SE (n = 4). *Significantly different from IgG control (P < .01).