Fig. 7.
Production of osteoclastogenic activity in ST2 mouse bone marrow cells in contact with α4-integrin.
Confluent CHO cells expressing α4-integrin or empty vector were fixed with 2.5% paraformaldehyde and ST2 cells (2 × 105/24-well) were cultured on the monolayer of the fixed CHO cells for 24 hours. Conditioned media of these cultures were assayed for osteoclastogenic activity in mouse bone marrow cultures. EV indicates empty vector.
