Fig. 8.
Effect of neutralizing antibodies to known osteoclast-activating cytokines on bone-resorbing activity of conditioned medium harvested from co-cultures of 5TGM1 myeloma cells and ST2 mouse bone marrow stromal cells.
Conditioned medium (CM) was treated with 10 μg/mL neutralizing antibodies to IL-1α and IL-1β, IL-6, TNFα, TNFβ, and PTH-rP (20 μg/mL)22 for 1 hour at room temperature. Subsequently, antibody-treated conditioned medium was assessed for its bone-resorbing activity in the fetal rat long bone assay as described in Figure 5.45Ca release in control culture medium was 17 ± 1% (not shown). Data are expressed as mean ± SE (n = 4).
