Efficient gene transfer and transgene expression by HIV-1 vectors in activated human lymphocyte subsets.

Transduction of PHA/rhIL-2–stimulated lymphocytes was carried out as described in “Materials and methods.” Relative gene transfer efficiency in T-cell subsets in the presence (+) or the absence (Δ) of APs were determined by analyzing the number of EGFP positive cells 5 days after transduction by FACS; cells were stained with the indicated PE-conjugated mAbs. Data shown are representative of similar data obtained from 2 independent experiments.