HIV-1 vector-mediated transduction does not alter the activation status or cell cycle progression of resting human PBLs.

(A) Relative gene transfer efficiencies of HIV-1 vectors generated in the presence (+) or the absence (Δ) of HIV-1 APs as determined by dual analysis of EGFP and activation marker expression by FACS. Cells were analyzed 5 days after transduction. Data shown are representative of 2 independent experiments. (B) Cell cycle status of adherent cells- depleted primary human PBLs before and 5 days after transduction with HIV-1-based vectors in the presence (+) or the absence (Δ) of HIV-1 APs by PI staining and flow cytometry. Percentages of cells residing in each phase of the cell cycle are indicated.