American Society of Hematology

Fig. 3.

$Fig. 3. IR-induced PFN expression in KG1a cells. / (A) Flow cytometry analysis of IR-induced PFN expression as determined on KG1a cells. Experiments were performed by flow cytometry with specific anti-PFN on KG1a irradiated (dashed line), or not (dotted line). Each histogram is representative of four different experiments. Irrelevant isotopic murine MoAb (black line) was used as internal control (au: arbitrary units). (B) IR-induced PFN expression in nuclear (pellet fraction) and cytoplasmic (supernatant fraction) lysates in KG1a cells. Western blot analysis of nuclear and cytoplasmic fractions from KG1a was performed using MoAb-detecting PFN (65 kDa; diluted 1/200). For each lane, the nuclear and cytoplasmic lysates were loaded with the same number of cell equivalents.$

IR-induced PFN expression in KG1a cells.

(A) Flow cytometry analysis of IR-induced PFN expression as determined on KG1a cells. Experiments were performed by flow cytometry with specific anti-PFN on KG1a irradiated (dashed line), or not (dotted line). Each histogram is representative of four different experiments. Irrelevant isotopic murine MoAb (black line) was used as internal control (au: arbitrary units). (B) IR-induced PFN expression in nuclear (pellet fraction) and cytoplasmic (supernatant fraction) lysates in KG1a cells. Western blot analysis of nuclear and cytoplasmic fractions from KG1a was performed using MoAb-detecting PFN (65 kDa; diluted 1/200). For each lane, the nuclear and cytoplasmic lysates were loaded with the same number of cell equivalents.

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