American Society of Hematology

Fig. 6.

$Fig. 6. Effect of antisense oligonucleotides directed toward GrB. / (A) KG1a cells were incubated with antisense GrB or sense (control) oligonucleotides for 14 hours, irradiated, cultured for 72 hours, and then co-incubated with K562 cells as targets. Cytotoxicity was measured by using a 4-hour nonradioactive LDH-release assay at 40:1 E:T ratio. Results are the mean ± standard deviation of three independent experiments performed in triplicate. *P = .02. (B) Western blot analysis of antisense or sense oligonucleotides on GrB expression after irradiation and cellular fractionation. IR: irradiation, S: sense, AS: antisense.$

Effect of antisense oligonucleotides directed toward GrB.

(A) KG1a cells were incubated with antisense GrB or sense (control) oligonucleotides for 14 hours, irradiated, cultured for 72 hours, and then co-incubated with K562 cells as targets. Cytotoxicity was measured by using a 4-hour nonradioactive LDH-release assay at 40:1 E:T ratio. Results are the mean ± standard deviation of three independent experiments performed in triplicate. *P = .02. (B) Western blot analysis of antisense or sense oligonucleotides on GrB expression after irradiation and cellular fractionation. IR: irradiation, S: sense, AS: antisense.

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