Expression of CD8α protein and mRNA in migratory LC.

(A) DC were isolated from the draining LN 1 day after FITC painting and were stained with PE-conjugated CD8 antibody. FITC⁺CD8⁺ cells were purified using a cell sorter and were analyzed by confocal microscopy. Magnification, 60×. (B) FITC⁺CD8⁺CD11c⁺cells (lane 1), FITC⁻CD8⁺CD11c⁺ cells (lane 2), and FITC⁺CD8⁻CD11c⁺ cells (lane 4) were sorted to more than 90% purity from a DC-enriched population isolated from the draining LN of FITC-painted mice and were subjected to RT-PCR analysis. A suspension of total LN cells (lane 3) served as positive control. For all samples, synthesis of cDNA was controlled by RT-PCR using β-actin primers for 30 cycles. One of 3 experiments with identical results is shown.