CD8⁺ LC are localized in the T-cell area of LN.

(A) Twenty-four hours after skin painting with FITC, draining LN were harvested, sectioned, fixed in acetone, and stained with biotin-labeled anti-CD8 antibody and then by streptavidin–PE to determine the localization of the CD8⁺ LC. Tissue was analyzed by confocal microscopy. Red cells represent CD8⁺ T cells. Most FITC⁺ cells (yellow) coexpress CD8 and are present in the T-cell region. Magnification, 10×. Adjacent cryostat sections (5 μm) stained with anti-CD4 revealed the CD4⁺ T cells to be localized in the same regions as the CD8⁺ cells (data not shown). (B) Adjacent tissue sections were fixed in methanol, air dried, and stained with hematoxylin and eosin to orient the reader to the normal architecture of the LN. Stained sections were analyzed using a bright-field microscope. Magnification, 10×.