CD8⁺ LC in LN, but not in skin, express the chemokine receptor CCR7 and the T-cell attracting chemokines MDC and TARC.

LC were isolated from ear skin as described in “Materials and methods,” and FITC⁺CD8⁺CD11c⁺cells were isolated from the LN of painted mice. The presence of CCR6, CCR7, MDC, and TARC mRNA was assayed by RT-PCR using specific primers. For each sample cDNA synthesis was controlled by RT-PCR using β-actin primers for 30 cycles. Results of 1 of 3 representative experiments are shown.