Competition of FANCA/FANCG binding by the 20 amino acid peptide from the NLS region of FANCA.

(A) ³⁵S-labeled, in vitro-translated FANCG(wt) protein was preincubated with the indicated concentrations of FANCA-NLS-wt peptide or FANCA-NLS-mut2 peptide for 30 minutes. ³⁵S in vitro-translated, FANCA 1-240 was subsequently added, and an immunoprecipitation was performed with an anti-FANCG antiserum. (B) The autoradiograph in (A) was subjected to densitometric scanning and plotted. FANCA/FANCG binding was normalized as the ratio of HA-FANCA 1-240 over FANCG. The binding in the absence of peptide was set as 100%.