The amino terminal region of FANCG is necessary but not sufficient for functional complementation of an FA-G cell line.

The FA-G lymphoblast line, EUFA316, was transfected with cDNAs encoding various forms of the FANCG protein. G418-selected cells were analyzed for the function of the various FANCG mutant proteins, using the MMC assay. Samples shown are parental EUFA316 cells (○), EUFA316 cells transduced with FANCG(wt) (●), with FANCG 1-428 (▵), with FANCGΔexon13 (■), or with FANCG1749ΔA (♦). Similar results were obtained when the FANCG cDNAs were transfected into the FA-G fibroblast line, FAG326SV (data not shown).