Truncated mutant forms of the FANCG protein bind to FANCA but not to FANCC.

The FA-G lymphoblast line, EUFA316, expressing the various indicated forms of the FANCG protein, was analyzed for FANCA/FANCC/FANCG binding. Proteins extracted from the indicated EUFA316 transfectants were immunoprecipitated with an anti-FANCA antiserum, and the immune complexes were analyzed by immunoblotting with either anti-FANCA, anti-FANCG (N-terminal), or anti-FANCC antisera. In lane 2, IP-FANCA/Blot-FANCG, FANCG 1-428 migrated slightly faster than immunoglobulin heavy chain. Alternatively, whole cell extracts (WCE) were immunoblotted directly.