Fig. 5.
Fig. 5. Confocal microscopy and double immunostaining of Egr-1 and PECAM in the liver of VEGF- and EGF-treated mice. / Mice received intraperitoneal injections of normal saline, VEGF, or EGF. Livers were removed 1 hour later and processed for immunodetection of Egr-1 and PECAM, as described in Figure 4. (Top) Low-power view of optical sections through the liver of normal saline-treated animal (A-C), VEGF-treated animal (D-F), and EGF-treated animal (G-I). Egr-1 immunofluorescence is shown on the left (A, D, G), and PECAM immunofluorescence is shown in the middle (B, E, H). The 2 images are combined on the right (C, F, I). Bar, 100 μm. (Bottom) High-power view of optical sections through the liver of VEGF-treated animal (A-C) and EGF-treated animal (D-F). Egr-1 immunofluorescence is shown on the left (A, D), and PECAM immunofluorescence is shown in the middle (B, E). The 2 images are combined on the right (C, F). Bar, 25 μm.

Confocal microscopy and double immunostaining of Egr-1 and PECAM in the liver of VEGF- and EGF-treated mice.

Mice received intraperitoneal injections of normal saline, VEGF, or EGF. Livers were removed 1 hour later and processed for immunodetection of Egr-1 and PECAM, as described in Figure 4. (Top) Low-power view of optical sections through the liver of normal saline-treated animal (A-C), VEGF-treated animal (D-F), and EGF-treated animal (G-I). Egr-1 immunofluorescence is shown on the left (A, D, G), and PECAM immunofluorescence is shown in the middle (B, E, H). The 2 images are combined on the right (C, F, I). Bar, 100 μm. (Bottom) High-power view of optical sections through the liver of VEGF-treated animal (A-C) and EGF-treated animal (D-F). Egr-1 immunofluorescence is shown on the left (A, D), and PECAM immunofluorescence is shown in the middle (B, E). The 2 images are combined on the right (C, F). Bar, 25 μm.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal